The data system computer with the q exactive tune software is switched on and the mass spectrometer is placed in standby mode. We are located in the lammot du pont laboratory and provide analyses for the department of chemistry and biochemistry as well as the entire university community. This instrument is a high resolutionmass accuracy orbitrap mass spectrometer with maximum resolving power of 120,000 or mass accuracy jul 11, 2017 incredible resolving power up to 120,000 fwhm for the q exactive gc at mz 200 and up to 50,000 fwhm for the exactive gc at mz 272, subppm mass accuracy with a high acquisition rate, driving unrivaled spectral quality. For information about performing these tasks, refer to the exactive series operating manual, the qexactive software plus manual or the q exactive plus tune help, and the ion max and ion maxs api source hardware manual. Trust your foods are all they should be food adulteration and. Thermo trace gc ultra operating manual the isq gcms series features a new source design ideal for continuous trace 0 gc, trace 10 gc, thermo scientific triplus rsh. This is a stateoftheart hybrid quadrupoleorbitrap high resolution msms instrument and can be used for the analysis of a wide range of biochemical species and comes equipped with a wide variety of capabilities including. To explore the interaction, we used a novel gc orbitrapbased mass spectrometer, the q exactive gc, which combines the high peak capacity and chromatographic resolution of gas chromatography with the subppm mass accuracy of an orbitrap system. The exactive gc system is an easytouse, dedicated gcms that provides an unprecedented level of highly sensitive, routine grade performance for both targeted and nontargeted analysis, along with high confidence quantitation for the ultimate sample analysis workflow.
Q exactive gc quickstart guide thermo fisher scientific. The structure of q exactive hybrid quadrupoleorbitrap mass spectrometer as shown in figure 2, the q exactive hybrid quadrupoleorbitrap mass spectrometer mainly includes an ion source, a stackedring ion guide slens, a quadrupole mass filter, a curved linear trap ctrap, a higher energy collisional dissociation hcd cell, and an orbitrap. Aias 10 user manuals aias 3000 user manuals triplus rsh user manuals triplus 100 ls user manuals triplus 300 headspace user manuals triplus classic user manuals trace 0 series gc user manuals isq series manuals tsq series manuals legacy ms manuals. And the exactive gc orbitrap gc ms system brings the power of highresolution, accuratemass hram analysis into the routine environment for the first time. Apr 03, 2017 q exactive gc deconvolution plugin software manual. Chose file load tune file to display the open dialog box. This easytouse system provides the most comprehensive characterization of samples in a single analysis for the highest confidence in compound discovery, identification, and quantitation. In addition, this manual provi des stepbystep instruct ions for cleaning and maintaining your.
With bestinclass performance characteristics for both quantitative and discovery workflows, the q exactive gc system allows the most comprehensive characterization of complex samples. The exactive gc orbitrap gc delivers sensitive, routine grade performance in both targeted and. With a fast scan speed and multiplexing capabilities, the q exactive mass spectrometer is an outstanding detector for. Both mass spectrometers were evaluated for key analytical parameters such as scan speed, sensitivity, mass accuracy, dynamic range and linearity. For more information about the tracefinder software application, please refer to the tracefinder user documentation. The q exactive lcmsms system not only offers broad screening capabilities but also. The quality of data from q exactive allows identification and quantification of more compounds with outstanding confidence. High selectivity fullscan analysis brings new possibilities to the routine environment.
Thermo scientific q exactive gc preinstallation requirements guide pn 1r1207060001, revision b 11 chapter 1 using this manual welcome to the thermo scientific q exactive gc system. Screening and quantitation using high resolution accurate. Log in your ass spec qexactive session on the cores. Please refer to exactive gc specification sheet ps10506 and exactive gc technical note tn10511. Q exactive gc orbitrap gcmsms system planet orbitrap. The exactive plus mass spectrometer is a standalone orbitrap. This exactive software manual is intended for all personnel that need to perform measurements with the exactive mass spectrometer, especially the key operator. In addition, this manual provi des stepbystep instruct ions for cleaning and maintaining your instrument.
For information about performing these tasks, refer to the q exactive gc and exactive gc operating manual, the exactive gc software manual or the exactive gc tune help. If you have not already done so, choose start programs thermo exactive exactivetune to start the exactive tune program. Gasphase chemistry in the gc orbitrap mass spectrometer. This guide provides reference information about the parameters in the thermo scientific deconvolution plugin application within thermo scientific tracefinder software for gas chromatography gc and mass spectrometry ms instruments. Ionbench with the shimadzu 8080 mass spectrometer nexera. Food safety analysis and testing solutions offer exceptional. The thermo scientific q exactive gc hypbrid quadrupoleorbitrap gc msms system represents the firstever combination of highresolution gas chromatography and highresolution accuratemass hram orbitrap mass spectrometry. Exactive series installation request form please refer to the exactive series prei nstallation requirements guide pn 1288110, re vision a for the complete site requirem ents. Thermo scientific q exactive gc hybrid quadrupoleorbitrap mass spectrometer. Getting ions from experiments this topic describes how to test the instrument before you can calibrate your exactive gc mass spectrometer automatically. Upgrade your maps peptide mapping thermo scientific. Gc ms low resolution ei only using the agilent 5973 system. Q exactive tandem mass spectrometry lcmsms the q exactive hybrid quadrupoleorbitrap mass spectrometer from thermo scientific is an lcmsms system combining quadrupole precursor ion selection with highresolution, accuratemass orbitrap detection. The spectrum window of exactive gc tune shows a realtime display of the mass spectrum.
Can anyone help with an orbitrap mass spectrometer. This manual should be kept near the instrument to be available for quick reference. This is achieved through the superior resolving power, mass accuracy, linear dynamic range and sensitivity that only orbitrap technology can deliver, combined with the intelligent data processing workflows provided by thermo. Thermo trace gc ultra operating manual the isq gc ms series features a new source design ideal for continuous trace 0 gc, trace 10 gc, thermo scientific triplus rsh. Exactive operating manual thermo fisher scientific.
Exactive quickstart guide thermo fisher scientific. Lcms and lcmsms using shimadzu lcms 2020, waters q tof and thermo q exactive orbitrap with electrospray ionization esi and atmospheric pressure chemical ionization apci. Instrumentation high resolution mass spectrometry facility. The exactive gc analyzer is an easytouse, dedicated gc ms system that provides an unprecedented level of highly sensitive routinegrade performance for both targeted and nontargeted analysis, along with high confidence quantitation for the ultimate sample analysis workflow. The new thermo scientific q exactive gc hybrid quadrupoleorbitrap gc msms newgeneration system redefines performance for gc ms applications. In the exactive tune window, on the toolbar, click the onoffstandby button to take the ms detector out of the standby mode or off mode and turn it on. This text is a guide to operating the qexactive mass spectroscopy. The resolution was set to 120,000 fwhm throughout analysis of liquid samples under varying conditions, and for spme samplesofxcn resolutionwas set to 60,000fwhm. Thermo scientific content manual and documents official. The thermo scientific q exactive gc hybrid quadrupole orbitrap mass spectrometer provides an unmatched combination of sensitivity, massaccuracy and.
Q exactive gc operating manual pn 1r1207060002, revision a i. We are also a core facility of the delaware biotechnology institute. A q exactive gc mass spectrometer thermo fisher scientific, bremen, germany was used in all experiments. See the q exactive gc and exactive gc operating manual for instructions to configure the ci gas type installed at. The q exactive gc system brings together qualitative and quantitative analyses by combining the highprecision, full scan hram capability of orbitrap technology with the quantitative power. The q exactive benchtop lcmsms combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection to deliver high performance and tremendous versatility. The thermo scientific q exactive gc hybrid quadrupole orbitrap mass spectrometer provides an unmatched combination of sensitivity, massaccuracy and resolving power in a single analysis for the highest confidence in compound discovery, identification, and quantitation. The thermo scientific tsq triple quadrupole gc msms systems are the gold standard for the sensitive and specific quantitation of target compounds. Q exactive gc orbitrap gcmsms thermo fisher scientific. This q exactive gc operating manual contains precautionary statements that can prevent personal injury. This easytouse system provides the most comprehensive characterization of samples in a single analysis for the.
This instrument is capable of automated liquid and headspace gas sampling, and solid phase microextractions spme for ms analysis with electron or. We now have a commercial version of this initial system that offers routine metabolite profiling at high mass accuracy owing to the high field orbitrap mass analyzer in the system. A thermo q exactive was installed in january 2020 and purchased with funds from the nsf major research instrumentation and the chemical instrumentation programs 1919422. Waters 3100 waters acquity sqd waters xevo tq ms waters xevo tqs waters xevo tqd waters xevo g2 tof waters xevo g2 q tof waters xevo g2xs qtof waters xevo tqs micro. To set up the mass spectrometer in the q exactive tune software for tuning and calibration in the hesims mode 1. Q exactive gc and exactive gc operating manual pn 1r1207060002, revision b. Maldi analysis of proteins, oligonucleotides, nanoparticles, synthetic polymers and macromolecules.
Product specifications thermo scientific q exactive benchtop. The exactive gc system is an easytouse, dedicated gc ms that provides an unprecedented level of highly sensitive, routine grade performance for both targeted and nontargeted analysis, along with high confidence quantitation for the ultimate sample analysis workflow. Thermo q exactive gc orbitrap gc ms thermo tsq altis ms thermo tsq quantis ms thermo q exactive hfx thermofisher exploris 120 thermofisher exploris 240 thermofisher exploris 480. The q exactive a benchtop orbitrap mass spectrometer. Thermo q exactive hybrid quadrupoleorbitrap gc ms the gc orbi is used for the identification and quantification of small molecules at high chromatographic and mass resolution and high sensitivity. The q exactive hybrid quadrupoleorbitrap mass spectrometer from thermo scientific is an lcmsms system combining quadrupole precursor ion selection with highresolution, accuratemass orbitrap detection. This will include the latest results from a proofofconcept study using the q exactive gc for the analysis of gc amenable pesticides and the results from a similar study using lc q exactive. Using vev on the q exactive gc and exactive gc gc ms systems can allow for enhanced sensitivity for more specific high mass ions the automated tuning system greatly reduces complexity and improves operational efficiency for optimizing sensitivity at electron energies ranging from 10 and 150 ev. The q exactive is an orbitrap mass spectrometer capable of very high resolution measurement of masstocharge ratios and tandem mass spectrometry msms, that will support. The new thermo scientific q exactive gc hybrid quadrupole.
This instrument is a high resolutionmass accuracy orbitrap mass spectrometer with maximum resolving power of 120,000 or mass accuracy q exactive gc orbitrap gcms system. Orbitap gc ms systems the thermo scientific q exactive and exactive gc orbitrap gc ms systems represent the firstever combination of capillary gas chromatography and high. The superior quality of q exactive msms data enables identification and quantitation of more compounds with greater confidence. Due to this tight association we hypothesised that metabolic effects were also in evidence. Q exactive gc mass spectrometer software manual thermo fisher. Thermo scientific qexactive orbitrap mass spectrometry. Instrumentation fraternal order of eagles diabetes research. The exactive gc system provides the same level of performance when operated under the same conditions. The exactive gc system has the quantitative power of a gc triple quadrupole ms combined with the fullscan highresolution, accuratemass hram capability that only orbitrap gcms technology can offer. The q exactive gc orbitrap is a relatively easytouse gc ms system that provides high quality data for compound discovery, identification and quantitation for comprehensive analyses. If you have not already done so, choose start programs thermo exactive series tune to start the q exactive plus tune program. At present we have a wide variety of instruments using ei, ci, fd. Instrumentationinstruments used were the q exactive orbitrap gc system, including a q exactive orbitrap mass spectrometer connected to a trace 10 gc equipped with a triplus rsh autosampler thermo scientific, bremen, germany and the gc isq system, including a isq singlequadrupole mass spectrometer connected to a.
Exactive is a member of the thermo scientific fa mily of advanced mass spectrometer ms detectors. The q exactive gc system is a member of the thermo scientific family of mass spectrometer ms detectors that are powered by orbitrap technology. I am using a q exactive plus and in the experiment pane for a full ms ddms2 topn there are three settings for the automatic gain control agc, the number of ions to be in the detector orbitrap. Q exactive gc mass spectrometer the q exactive gc tuned and calibrated using fc43 to achieve m ass accuracy of q exactive gc orbitrap gc ms thermo tsq altis ms thermo tsq quantis ms thermo q exactive hfx thermofisher exploris 120 thermofisher exploris 240 thermofisher exploris 480. Instrumentation fraternal order of eagles diabetes. Gas chromatography mass spectrometer gc ms isq 7000 single quadrupole gc ms system. About this guide this exactive operating manual describes the modes of operation and principle hardware components of your exactive instrument. The system can be coupled with either an agilent 1260 infinity capillary pump with thermostatted column compartment and autosampler or a. Gcorbitrap for food safety analysis separation science. Metabolomics laboratory instruments nyu langone health.
The objective of this study was to test the analytical performance of the exactive gc system and the q exactive gc system using fullscan acquisition. The exactive family the exactive plus hcd cell optional upgradeable to q exactive mass range to mz 6000 faster polarity switching rotated flatapole new scan modes the q exactive resolving power 140,000 parent ion selection capability spectral multiplexing uhplc and nanoflow capable. Exactive gc mass spectrometer shutdown, startup and reset. Documents this large are best viewed by clicking the view pdf link. Instrumentation ucla pasarow mass spectrometry laboratory.
Bring the power of the firstever combination of highresolution gas chromatography gc and highresolutionaccuratemass hram orbitrap mass. In the q exactive plus tune window, on the toolbar, click the onoffstandby button to take the mass. Trust your foods are all they should be food adulteration. This guide should be retained for future guidance if your instrument needs to be relocated in future. There are several ways to contact thermo fisher scientific. A more detailed and thorough account of these procedures can be found in the qexactive operating manual. The q exactive gc orbitrap gc msms system is here with the superior resolving power, mass accuracy and sensitivity that only thermo scientific orbritrap technology can deliver. Q exactive gc deconvolution plugin software manual. Q exactive hybrid quadrupoleorbitrap mass spectrometer. Thermo q exactive mass spectrometer a thermo q exactive hram orbitrap mass spectrometer combines quadrupole precursor selection with high resolution providing high performance and versatility. Exactive gc tune downloads the tune method parameters to the. Scientists have long demanded more gc ms power for detailed q exactive gc orbitrap gc msms with autosampler trace gc columns for pesticides. Exactive plus operating manual pn 23060, revision a.
Q exactive gc our laboratory coupled the first gc to an orbitrap analyzer in 2010. Who uses this guide this exactive gc software manual is intended for all personnel that need to perform measurements with the exactive gc mass spectrometer, especially the key operator. The thermo scientific q exactive gc hypbrid quadrupoleorbitrap gcmsms system represents the firstever combination of highresolution gas chromatography and highresolution accuratemass hram orbitrap mass spectrometry. Dec 19, 2018 a q exactive gc mass spectrometer thermo fisher scientific, bremen, germany was used in all experiments. This is achieved through the superior resolving power, mass accuracy. A perfect match for the q exactive gc system, the thermo scientific triplus rsh autosampler utilizes robotic sample handling to expand automated capabilities beyond liquid, headspace, and solidphase microextraction spme injections to advanced sample handling cycles. Exactive gc tune preparation getting ions from experiments. Overview to scale the picture below is a simulation at scale of the thermo q exactive gc orbitrap gcms system spectrometer connected with a trace 0 gc, installed on a ionbench ms mass spec bench or table the vacuum pump leybold sv65bi is enclosed in the noise reduction cabinet, under your mass spectrometer thermo q exactive gc. The thermo scientific q exactive gc hybrid quadrupoleorbitrap mass spectrometer provides sensitive and highresolution ion detection with quadrupole isolation and hcd fragmentation. With a fast scan speed and multiplexing capabilities, the q exactive mass spectrometer is an outstanding detector for fast chromatography separation techniques.
The exactive gc orbitrap gc delivers sensitive, routine grade performance in both targeted and nontargeted screening protocols. The thermo scientific q exactive gc hybrid quadrupole orbitrap mass spectrometer provides an unmatched combination of sensitivity, massaccuracy and resolving power, helping answer todays most challenging analytical questions. When you turn the ms detector to on, you initiate the following events. Scope of this guide the exactive software manual includes the following chapters. Product specifications thermo scientific q exactive. A novel targeteduntargeted gcorbitrap metabolomics. Our experience has shown us that the q exactive gc definitely brings a new level of performance into gc highresolution mass spectrometry, said hajslova. The thermo q exactive gc qe gc is a hybrid quadrupoleorbitrap mass spectrometer with a trace 10 gas chromatograph gc. Scientists have long demanded more gcms power for detailed q exactive gc orbitrap gcmsms with autosampler trace gc columns for pesticides. To set up the ms detector in the exactive tune software for tuning and calibration in the esims mode 1. About this manual this q exactive gc preinstallation requirements guideis intended primarily for those who are responsible for the site planning of a laboratory in preparation for the installation of a new exactive series instrument. New system redefines performance for gcms applications. The q exactive a benchtop orbitrap mass spectrometer for dmpk presented to.
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